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Consequently, the transgenic lines showed a significant enhancement in total seed lipid content ranging between 7.5 and 16.9%.
Metabolic analysis of RNAi-AH1 hairy root lines showed a significantly increased accumulation of miltiradiene compared to the control lines.
The selected cell lines showed a tight regulation of the expression system with the appearance of Rho protein 6 h following induction (Fig. 3b).
Without mannitol treatment, WT and transgenic lines showed a similar germination rate.
In the third generation, four lines showed a significant deficit of heterozygotes, while two lines showed a significant excess of heterozygotes (mean I = −0.02±0.043; Figure 2c).
Other lines showed a response within 48 hours (Figure S6, Table S1).
Both lines showed a complete elimination of IA (data not shown).
Surprisingly, none of the mutant lines showed a short root phenotype or downy mildew resistance.
Notably, BRAFV600E mutant melanoma cell lines showed a limited sensitivity in response to low energy conditions.
The two cell lines showed a higher expression of α6 integrin compared with primary cells.
The other 11 cell lines showed a wide range of sensitivities.
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