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Depending on whether mainlines use their own or third party common feeder carriers, the hub port selection decision can be affected by the shipping lines' preference for hub ports with highly connected feeder services.
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All the below-the-line preference data was then combined with the registered preferences for above-the-line votes and fed into a computer program that distributed the preferences and determined who would be elected.
Thus, all investigated mutations change the product line preference by shifting APP processing towards the Aβ38 production line.
Inhibition of PI3K reduced rhTRAIL sensitivity independently of the cell line preference for either DR4- or DR5-mediated apoptosis signaling.
A neo-epitope antibody against AICD50 99 (characterized in Supplementary Figure 3) was generated, and allowed us to confirm the product line preference.
Figure 4G actually shows that FAD APP mutations change the product line preference (Aβ40/Aβ38 ratio), but do not alter the fourth enzymatic turnover (Aβ38/Aβ42 ratio).
This result corroborates our claim that the product line preference is an intrinsic property of the γ-secretase complex that remains unaltered in our cell-free assay.
In agreement, it has been shown that the endoproteolytic cleavage site determines the product line preference of the γ-secretase in vivo (Funamoto et al, 2004), and therefore the series of Aβ products.
For households contacted by fixed-line, preference was given to women aged 21-39 years over those aged 18-20, and if there were still more than one, random selection was used.
The fact that some FAD PSEN1 mutations combine these two mechanisms (impaired fourth cycle and change in the product line preference) explains the direct and indirect correlations between Aβ38 and Aβ42 levels reported in the past (Czirr et al, 2008; Page et al, 2008).
A further proposal for reform is to allow above the line preferencing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com