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Correlations were performed using 160 RI lines of the population.
PC175 and other self-incompatible DH lines of the population were manually bud pollinated to get enough seed.
We observed several lines with a few heterozygous chromosomal regions, for which pollen contamination that occurred in the field between lines of the population is the most probable explanation.
Another 40% of the rye genotyping array 2.0 originated form DNA of parental lines of the population used in this study (mapping array 1.0) and 18.5% of them (851 markers) segregated in the RILs.
Creation of a single near-isogenic line generally starts by crossing a line carrying the targeted QTL region to one of the parental lines of the population, thus creating a backcross population.
For each of three separate sets, each of which were pooled for the construction of an Illumina sequencing library, 192 different BATs were used for PCR amplification to screen 1,536 lines of the population combined by one-dimensional 8-fold pooling.
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The methodology can be an effective support to increase reliability, save consumers' money and mitigate the unavoidable impacts of the lines on the population living nearby.
Comparison of phenotypic values between any line of the population and the recurrent parent generates high statistical power.
Similarly, reduced levels of polymorphisms were detected between CR Shinki and 94SK (14%, the parental lines of the CSKF2 population) and between 501 and 601 (15.56%, the parental lines of the PF2 population).
For example, Eckert et al. [24] only reached an 18.2% conversion rate in a P. taeda mapping pedigree, using in vitro SNPs from a database that did not include any sequences of the parental lines of the mapping population.
Histogram of the frequency distribution of root traits among RI lines of the mapping population (V71-370/PI407162).
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branch of the population
performance of the population
sector of the population
lines of the letter
lines of the program
lines of the flour
lines of the crisis
lines of the poem
lines of the aisle
lines of the cello
lines of the referendum
lines of the vehicle
lines of the study
lines of the user
lines of the night
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