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There was iPhone-ish lines, media coverage, the works.
Recent literature suggests that the protocols and conditions (time lines, media, serum supplement, etc).
For LNCaP and 22Rv1 cell lines, media were additionally supplemented with 1 mM sodium pyruvate, 4.5 g/l glucose, and 10 mM HEPES buffer (pH 7.2).
In order to establish stable cell lines, media supplemented with either 0.35 μg/ml puromycin (Sigma) or 500 μg/ml G418 (Invitrogen) were used.
Chemicals and reagents; all cell lines, media, trypsin-EDTA, fetal bovine serum (FBS) and antibiotics (penicillin, streptomycin and fungizone) were purchased from Highveld Biological (Pty) Ltd. (Modderfontein, Johannesburg, RSA).
All cell lines were maintained through continuous passaging, and were confirmed to be free of contamination by Mycoplasma spp. Cells were maintained in DMEM (ZR-75-1 celineine) or RPMI-1640 (other cell lines) media (Sigma-Aldrich, St . Louis MO, USA), with 10% FBS (Sigma-Aldrich), 100 U/mL penicillin, 100 μg/mL streptomycin (Gibco, Rockville, MD, USA) and 1.46 mg/mL L-glutamine (Gibco).
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