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Those datasets are narrow-based populations with a multifamily structure, each family being small (circular mating cross of 32 parents with an average of 13.5 individuals per family for the loblolly pine population and advanced breeding lines generated the same year for the Cornell wheat population).
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However, in the course of our work we have found that retroviral vector silencing in chimpanzee iPSCs was not as stable as in human iPSC lines generated at the same time using the same method (see 'Materials and methods' and Figure 1 figure supplement 5).
We also used hFIB2 hiPSC line (courtesy of George Daley [35] generated the same way as that for YZ1 and YK26.
The differences in tumor multiplicity and latency among transgenic lines generated with the same transgene construct might rest in the random integration of the transgene into the mouse genome, leading to different phenotypes and levels of transgene expression depending on the site of integration and on the number of transgene copies.
In order to carefully investigate the possible CNV instability during somatic reprogramming, we performed genome-wide CNV analyses with 41 mouse iPSC lines generated from the same parental donor; therefore, the donor's genetic background can be controlled.
It is a common observation that mouse lines generated with the same transgene construct can differ in their expression and show a mosaic pattern.
Marked differences in differentiation propensity between pluripotent stem cell lines, even between iPSC lines generated from the same individual, have been reported [ 53, 54].
In the present study, we applied exome sequencing on a series of isogenically-derived immortalized human colonic epithelial cell (HCEC) lines generated from the same individual with defined genetic manipulations.
In conclusion, to the best of our knowledge, this is the first report where a large complex genetic mutation has been mapped, patient fibroblasts successfully reprogrammed and a disease-specific phenotype observed in cell types differentiated from experimental hiPS cell lines compared with those from isogenic control hiPS cell lines generated from the same patient.
And in both places they generated the same reaction, the same enthusiasm, the same hope.
The line generated the Devils' first goal.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com