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The work was performed using cell lines from the Collection of Microorganisms and Cell Cultures of the Leibniz Institute DSMZ-German.
Therefore, expression data for these 28 cell lines from the collection could be used as biological replicates.
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If you have a very large tank and are collecting significant amounts of organic matter, try running a line from the collection cup to a separate tank.
One hundred twenty one inbred diploid lines from this collection were used in the present study (additional file 3).
To obtain the pools, we used DNA extracted from lymphoblastoid cell lines from the HapMap collection (obtained from the Coriell Institute for Medical Research).
An outbred population was created by cross-mating virgin females and males from 173 of the inbred lines from the RAL collection.
An outbred population was created by cross-mating virgin females and males from 173 inbred lines from the RAL collection (see methods).
Genomic DNA samples were obtained from established lymphoblast cell lines from the BPR collection.
The EY screen utilized 'empty UAS' insertion lines from the EY collection (50).
Additional stocks, including the lines from the CSS collection (Kurusu et al. 2008), are listed in Supporting Information, Table S1.
For our screen, we selected GAL4 lines from the entire collection that expressed in anterior sensory neurons.
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