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We chose 4 lines for study, all of which expressed Sulf-2 protein and one of which expressed both Sulfs.
To investigate the levels of activation of STAT3 in the treatment-responsive GCB lymphoma relative to ABC lymphoma, we selected eight patient tumor derived cell lines for study and examined levels of total and activated STAT3 by using immunoblot analysis.
Additional maize transformation vectors have been generated in which E1 is driven by other strong promoters and fused to alternative signal peptides to target accumulation in the vacuole and endoplasmic reticulum, and these constructs will be used to generate additional transgenic lines for study.
In selecting luminal/ER+ cell lines for study, one could conceivably use our scoring for PI3K signature activation as a guide, although it remains to be seen how cell lines with high PI3K scores might behave differently from cell lines with low scores.
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Still, stem cells made by nuclear transfer could have substantial advantages over existing stem cell lines for studying basic processes of human development.
Human Epithelial Kidney cell lines (HEK) are used as normal cell lines for studying effect of drug on non-cancerous cells within human body.
However, immortalisation disrupts the normal cell cycle regulatory mechanisms, such as phosphorylation of Rb protein, limiting the appropriateness of using immortalised lines for studying cell cycle mechanisms.
There are two major attributes of epiSCs that can be utilized to generate reporter lines for studying epigenetic reprogramming.
Human neuroblastoma cell line SH-SY5Y is one of the widely used cell lines for studying the neurodegeneration and neurotoxicity related to PD.
Various germplasms were successfully utilized for development of chromosome segment substitution lines for studies on pest and disease resistance and other agronomic triats in rice [ 21- 23].
Applying this method to individual pathways, we identified the appropriateness of particular cell lines for studying specific pathways in cervical cancer.
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