Knockdown of ATAD2 and MYC in seven endometrial and 21 breast cancer cell lines demonstrated that cell lines that were dependent on MYC also depended upon ATAD2.
Transfection experiments in HEK293 and A549 cell lines demonstrated that UOC/pAdTLRGD polyplexes were able to deliver the plasmid and transfect both cell lines.
Polymerase chain reaction and reverse transcriptase polymerase chain reaction analysis performed on individual transgenic lines demonstrated that the MB-1Trp gene was correctly integrated and transcribed into mRNA.
Another study using normal cell lines demonstrated that late-replicating regions are gradually demethylated as the cell divides, in contrast to early-replicating regions in which methylation is better maintained20.
Phenotypic comparison of these three mtDNA lines demonstrated that the NZB-129 heteroplasmic mice, but neither homoplasmic counterpart, had reduced activity, food intake, respiratory exchange ratio; accentuated stress response; and cognitive impairment.
Anticancer activity evaluation using seven cancer cell lines and two non-malignant cell lines demonstrated that several derivatives display significant anticancer activity and great selectivity index toward T-lymphoblastic leukaemia cells.
Cell lines demonstrated that proliferation levels of DLD1, DLD1/SV-rLuc Cells and DLD1/SV-FLuc cells were significantly same relative to non-tumorigenic cell lines [34, 35].
Consistent with these data, analyses of subcellular mRNA and ribosome distributions in multiple cell lines demonstrated that cytosolic protein mRNA-ribosome distributions were strongly correlated, whereas signal sequence-encoding mRNA-ribosome distributions were divergent.
Recent studies in human cell lines demonstrated that CRISPR/Cas9 system has a potential off-target cleavage in a sequence and position-dependent manner, since Cas9 protein can tolerate small numbers of mismatches between sgRNA and target DNA (Fu et al., 2013; Hsu et al., 2013).
The capability to produce A549-CEACAM5 celinesnes demonstrated that in principle A549 cells are able to express GPI-anchored CEACAMs on their cell surface.
Finally, intracytoplasmic staining and flow cytometry analysis of various hESC and hiPSC lines demonstrated that the entire contents of each 96-well consisted of 64 89% cardiac troponin I (TNNI3)+ cardiomyocytes (Figure 6).
