During the period when we were generating UAS-Psni transgenic flies, four independently produced lines became available from the Drosophila community.
As data from additional cell lines becomes available, some sites now declared constitutive could fail to meet the necessary criterion.
Although C57BL/6J embryonic stem cell lines became available in recent years, most transgenic mice had been generated using 129S6/SvEv embryonic stem cells.
Lee and his men stayed nearby the heights for several days, in case a weakness in the Union line became available for attack.
The culture supernatant was also used to inoculate DH82 cells when the cell line became available; 5 days after the inoculation, we observed a cytopathic effect of SFTSV in DH82 cells.
In addition to Gal4 driver-lines, many UAS-effector zebrafish lines have become available.
Since several laboratories are working on the production of fly models of RNA dominant diseases, a number of independent insertion lines should become available to the community for a systematic investigation to be undertaken.
As we understand more about gating mechanisms of plasmodesmata, inducible plasmodesmata opening lines may become available, allowing us to dissect the importance of distinct symplasmic domains that pre-condition differential turgor pressure.
In-vitro toxicology testing has gained attention during the last decade, as regulatory issues are limiting the use of animals for this purpose, and because many different mammalian cell lines have become available for screening purposes [ 18, 19].
