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The first 15 lines are free, and up to 10 more lines cost $40, with each additional line costing $4.
These RIL lines are free for public research purposes [ 42].
In addition, we have confirmed that all cell lines are free from Mycoplasma infection (example shown in Figure 1).
It is also important that cell lines are free from contamination with infectious agents such as mycoplasma, which can influence their biological behaviour and present a risk to handlers and animals (Ishikawa et al, 2006; Sung et al, 2006; Harlin and Gajewski, 2008).
T-DNA mutant lines were cleaned up by backcrossing to WT-Col, allowing heterozygotes for the insertion to self-pollinate, and then repeating the process for a second and third time to ensure that these lines are free of other T-DNA insertions.
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Other lines are freed too soon, making the rigging twang and the sails flog.
Instead of simply providing a way to call your reps, as many apps today do, Stance lets you pre-record your voice message to be left on the rep's voicemail when the phone lines are freed up.
So it was that Congress passed legislation instructing army officers not to send fugitives back into slavery and, later, declared that all fugitives coming into Union lines were free.
All cell lines were free from mycoplasma.
All cell lines were free of mycoplasma contamination.
All cell lines were free from mycoplasma as tested with the indirect Hoechst DNA staining method.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com