In addition, genotyping of single nucleotide polymorphism (SNP) was carried out at the Mutational Analysis Facility (MAF), Karolinska Institutet, Stockholm, for all lines analysed by array-CGH using the marker panel and analysis system introduced by Hannelius et al [21].
LP designed and performed most experiments with cell lines, analysed data and wrote the paper.
In support of this, DLC1 expression was decreased in 62% (8/13) cell lines analysed by quantitative PCR, including four lines without detectable genomic changes in this region.
With an EC50 of 2.6 5.5 mmol/L, glioblastoma cells were the most susceptible cancer cell lines analysed in this study.
CRTAP mRNA was abundantly expressed in LCL and the majority of NHL cell lines analysed, but at lower levels in Jijoye, AK31, AK2000, Elijah, MAK-I and BL2.
The fact that RASSF7 is required for growth may explain why its expression is not silenced in the cancer cell lines analysed above.
Of 15 bladder tumour cell lines analysed, three showed homozygous deletion of all or part of the PTEN gene, but none had mutations detectable by SSCP analysis.
The resultant cellular defect was identical in both of the patient cell lines analysed and manifested as a profound loss of mitochondrial OXPHOS complexes I and IV.
The inhibitory potency of both substances was very similar in all four cell lines analysed, with IC50 values of ~1 μM (Table 1).
This may be due to a position effect of the transgene insertion since all the transgenic lines analysed in the present study are independent transformation events.
The differences between the two studies may represent true differences between the tumours and cell lines analysed or may be a reflection of the small sample sizes used.
