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The plasmids were linearized, size fractionated, purified (QIAquick Gel Extraction Kit, Qiagen), and microinjected into fertilized FVB mouse oocytes.
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Endotoxin-free purified BAC vectors of >150 kb size were linearized and nucleofected into 0.5×10 or 1×10 PKC.
Some computational results show that the proposed model is more efficient than Oral Kettani's method because it uses less additional variables and auxiliary constraints to linearize the same size of mixed-integer problems.
Each reduced size class set is used to create a non-linear cumulative rates model which is linearized to design a linear model predictive controller.
The 5 Kbp and Lambda CHEF DNA Size Standard (Bio-Rad Laboratories, HerCAles, CA, USA) were used to estimate the size of the enzymatically linearized H. andersenii mtDNA.
The ditags were ligated overnight according to Kenzelmann and Mühlemann [ 33] to form concatemers, then partially digested with 2 U of NlaIII for 1 min at 37°C according to Gowda et al. [ 34] to create linearized clonable concatemers of suitable sizes.
The model is then linearized and this linear version is studied in depth, finding that the behavior of the suspension as reflected in the aforementioned three characteristics is strongly dependent on the size of the three suspension parts.
In so doing, Fujigaki's (1981) private goods economy is extended to involve a public good; and the intertemporal game of Champsaur and Laroque is piecewise localized by dividing the time interval in their game and by using variable step-sizes to formalize the piecewise linearized procedure, called λMDP Procedure, that can possess similar desirable properties shared by continuous-time procedures.
Treatment of agarose slices linearized the plasmid DNA and allowed for plasmid size determination using a concatomerized bacteriophage lambda standard (New England BioLabs, Beverly, MA) (5).
Variable step-sizes are used to formalize the piecewise linearized procedure that shares similar desirable properties with continuous procedures.
This treatment linearized approximately 20% of the YACs in the 100 400 kb size range.
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