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The linearized movement equations are derived and the stability of the equations is judged according to the initial equilibrium solution (IES).
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It linearizes the movement and the observation model to provide an effective online estimation.
In this paper, according to the mathematical model of the hydraulic step system in the flow field, the second-order nonlinear differential equations describing the step body movement are linearized by the local segmenting method, and then the simple numerical solution is obtained by Laplace transformation.
If the dynamic movement can be linearized at a working point of the mean deflection, a modification to the linear approach is suggested.
The targeting fragment was liberated and linearized in the female germline using FLP and I- SceI, respectively, and progeny were screened for movement of the mini-white marker to the second chromosome, as well as its resistance to eyFLP (indicating that it is no longer flanked by FRT sites, as in the donor).
The targeting fragment was liberated and linearized in the female germline using FLP and I- Sce I, respectively, and progeny were screened for movement of the mini-white marker to the second chromosome, as well as its resistance to eyFLP (indicating that it is no longer flanked by FRT sites, as in the donor).
WPN25-sfGFP were purified, linearized and analysed by PFGE.
This vector was subsequently linearized with PmeI and gel-purified.
All the plasmids used were linearized prior transfection.
pKO-AMA1 was linearized with PstI before transfection.
Ligate linearized product and annealed oligos at equimolar concentration.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com