Sentence examples for linearized expression from inspiring English sources

For stable expression in Dnmt1c/c ES cells, 5×106 Dnmt1c/c ES cells in PBS buffer were electroporated with 20 µg each of the linearized expression vectors encoding RFM mutants along with 2 µg each of linearized pPGK-puro using the BioRad Gene Pulser II (200 V, 500 µF).

A linearized expression vector construct, containing a cardiac-restricted ncx1 promoter driven puromycin resistance cassette and a phosphoglycerate kinase (pgk) promoter driven neomycin resistance (neoR) cassette, was electroporated into mouse R1 ES cells (Figure 1A).

DNA transfection was performed with 15 μg of linearized expression vector and 5 mg of an expression vector containing G418 resistant marker gene.

The linearized expression cassettes were transformed into P. pastoris wild-type CBS7435 based muts strain using standard protocols [ 84], and selected on zeocin™-containing plates.

P. pastoris/pPGKΔ3 α-L1H16 and P. pastoris/pPGKΔ3-L1H16 pastoris/pPGKΔ3-L1H16erecombinant after electroporation of yeastrainss were the linearized expressiobtainedttes, right pafter and furthelectroporation agar plates coftaining zeocin.

Cotransforming an S. pombe strain with the PCR product and the linearized expression vector produces plasmids with the PCR product inserted into the vector by gap repair (Kostrub et al. 1998).

The linearized expressions, on the other hand, are simple and allow for a straightforward analytical formulation of the ray tracing system.

MCF-10Atet cells were generated by transfecting MCF-10AecoR cells with the AhdI-linearized expression vector pWHE644, which uses the EF-1α promoter [ 49] to express a tri-cistronic transcript encoding the tetracycline-regulated transcriptional activator rtTA2S-M2, Tet-transsilencerncer tTSD-PP as well as a puromycin resistance gene [ 15].

The chicken cDNA encoding the NF-κB p100 [ 140] was released from the cloning vector pBS KS with HindIII and XbaI and inserted into HindIII and XbaI-linearized expression vector pBK-CMV (Agilent Technologies), resulting in pBK-CMV-p100.

We have also constructed a linearized BmNPV expression system, BmBacPAK6 [42] expressing a fusion gene including the HA gene of H5N1 (Hangzhou strain) [43] which is inserted the in-frame with the sequence that encodes the BmNPV gp64 signal peptide and transmembrane domain to create baculovirus Bmgp64HA.

The adapter sequences, which become incorporated into the termini flanking the amplified gene, are homologous to the cloning sites of the linearized T7 expression vector pXT7.