Finally, achieving non-linear transcription responses (multimeric factors, at least dimers, and/or multiple binding sites) also favor oscillatory behaviour.
The functional relationships may be "non-linear" because transcription factors may have a pleiotropic, cascading effect (Hadorn 1961).
The first is the sequence level, i.e. the linear organization of transcription units and regulatory sequences.
The linear decrease of transcription in the semi-log scale plot (Fig. 4B) during the DECREASE period indicated that the number gal transcripts decreased exponentially, suggesting first-order kinetics.
Specifically, log-expression levels of all genes present on the microarray were modeled as linear combinations of transcription factor activities.
We therefore developed a new protocol via a T7-based in vitro transcription linear amplification method that does not require PCR amplification.
The total RNA from single neurons were subjected to two rounds of linear T7 RNA Polymerase driven transcription using MessageAmp™ II aRNA Amplification Kit from Ambion.
According to our linear regression analyses, transcription of intronic proviral elements and solo LTRs, in general, appeared to be independent of their corresponding genes.
After generating the second strand, the complete cDNA was amplified by in vitro transcription (linear amplification) with a T7 RNA polymerase.
The RT primer in each TaqMan® miRNA assay is a single-stranded stem-loop RT primer which was developed to allow cDNA detection to be more specific and sensitive as compared to conventional linear primer reverse transcription [ 22].
