The coefficient α' in equations (6) and (7) can be considered as an equivalent radio-sensitivity, because it can be used to achieve same clinical results compared to more sophisticated linear – quadratic – proliferation models, as far as there exists a dose that can be considered uniform per fraction in RT.
As GGI is a linear combination of proliferation gene expressions (see Section 2.2), these results highlight the importance of proliferation measured by gene expression profiling in BC prognostication, and confirm the results of Sotiriou et al., (2006a, b).
Only the PS extract mediated an almost linear reduction in proliferation.
Radiological features of meningothelial proliferation (linear gadolinium enhancement and/or hydrocephalus) were present as early as 6 months and did not evolve over time.
Thus cell culture conditions were optimized for each cell line in order to treat cells when RTCA cell index reached 0.5 1 for HepG2 and 0.5 1.5 for HuH7 cells one day after plating, that is, in linear phase of proliferation.
Plasmids were transformed into the BL21 (DEMD Biosciencesences, Inc., San Diego, CA, USA) strain of Escherichia coli for expression and were induced with isopropyl-beta-D-thiogalactopyranoside after cells reached the linear phase of proliferation.
The MTT results showed that the viability of FLSs treated with KIAA1199 siRNA decreased significantly after KIAA1199 knock-down and increased after KIAA1199 over-expression; significant differences were observed between every time point examined, indicating a linear change in proliferation with treatment time, P <0.05.
Linear regression analyses of proliferation (KI-67), estrogen receptor (ER) status, and metabolite levels.
Using ACEA Biosciences RTCA software, version 1.2.1 (http://www.aceabio.com/product_info.aspx?id=187), we collected cell impedance data every 10 min to calculate doubling times from the slope of the linear phase of the proliferation curves.
Furthermore, after 48 hours of exposure with IOSi-NPs, we observed a tendency of linear increase of viability and proliferation.
