Sentence examples for linear polyubiquitination of two from inspiring English sources
It required linear polyubiquitination of two specific lysine residues of IRF-3 by LUBAC, the linear polyubiquitinating enzyme complex, which bound IRF-3 in signal-dependent fashion.
Detailed biochemical analyses revealed that a non-destructive linear polyubiquitination of two specific lysine residues (193, 313/315) of human IRF3 is sufficient to confer its RIPA activity; a minimal IRF3 mutant, harboring only those two specific lysine residues can activate RIPA.
The mechanism of IRF3 activation in RIPA is distinct from that of transcriptional activation; it requires linear polyubiquitination of specific lysine residues of IRF3.
Investigation of linear polyubiquitination of RIP1 during necroptosis revealed no differences between cpdm and WT MEFs (Supplementary Figure S8A).
Again, we observed significant K63 and linear polyubiquitination of RIP1 but not of RIP3 or caspase-8.
To examine the role of LUBAC in necrosome-associated linear polyubiquitination of RIP1, we knocked down HOIP and HOIL1.
Furthermore, a peptide signature consistent with linear polyubiquitination was detected in the hmCD40 sample (two spectra matching the peptide GGMQIFVK with approximately 80% confidence).
K63 and linear polyubiquitination function in the activation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) signaling pathway (Chen 2005, Terzic et al. 2007, Iwai & Tokunaga 2009).
We did not detect the other major component of the linear polyubiquitination complex, a protein known as heme-oxidized IRP2 ubiquitin ligase-1 (HOIL-1), in our mass spectrometry samples.
Linkage through the amino-terminus results in linear polyubiquitination that has recently been demonstrated to be a key step in nuclear factor κB activation; however, tools to study linear chains have been lacking.
Our in vitro assay largely recapitulates the K63 polyubiquitination-dependent pathways, but not the linear polyubiquitination-dependent pathway.
