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To begin, a general discussion on fabric descriptors employing linear intercept measurements is provided and a new fabric measure, referred to as areal pore size distribution, is proposed.
Mean linear intercept measurements were obtained using modified Image J software available from the National Institute of Health website (http://rsb.info.nih.gov/ij/).
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Quantification using mean linear intercept measurement (Fig. 6 D ) indicated that airspace enlargement in the DKO reaches significance in the lungs of 12 wk- and 18-mo-old mice.
Recently, McDonough et al. used micro-CT scans to quantify the degree of emphysema in different regions of lungs from patients with severe COPD by measuring the mean linear intercept (Lm), a morphological measurement of alveolar destruction [ 12].
Moreover, in our study there was a discrepancy between linear intercept and lung function measurements to detect emphysema after 3 months of CS exposure, with linear intercept being less sensitive than lung function technique.
Mean linear intercept (Lm) was calculated from measurements of the lengths of airspaces (both alveoli and alveolar ducts, but excluding airways) falling on top of these lines.
Physiologically, the nCB challenge-induced enlargement of the alveolar spaces concomitant with significant increases in total lung volume quantified by micro-CT imaging and unbiased lung morphometry measurement (mean linear intercept; MLI), both hallmarks of emphysema.
To determine the differences in alveolar size, measurements of mean linear intercept (Lm) were used.
Airspace enlargement was quantified by measuring mean linear intercept (Lm).
To further evaluate alveolar development, we measured the mean linear intercept (Lm; Fig. 2C).
The quantitative CT lung volume measurements in this protocol have been validated independently using mean linear intercept (MLI) data (Shan et al. 2012, Shan et al. 2014, Yuan et al. 2015).
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