a Mean linear intercept (Lm), fraction area of hyperinflated and collapsed alveoli.
To further evaluate alveolar development, we measured the mean linear intercept (Lm; Fig. 2C).
Standard stereological sample preparation, sectioning, and imaging of mouse lung tissues were performed for semi-automated acquisition of mean linear intercept (Lm) data.
The mean linear intercept (Lm), a measure of the surface area to volume ratio, is by and large the most commonly reported metric of emphysema.
Analysis of the mean linear intercept (Lm) revealed changes on the average size of alveoli in 129/Sv heterozygotes (Figure 3E).
Alveolar complexity of the lung was assessed by counting the number of individual saccules in each field, and measuring the mean linear intercept (Lm) (distance between alveolar walls).
The mean linear intercept (Lm) was measured on the lung section images using Image-Pro® Image-Pro®a Cybernetics, Bethesda, MD) image analysis software as described Plusiously [26]; see Figure 2. Briefly, a binary threshold Media Cyberneticslar septa was made, a grid of 5 cycloid lines was placed on the mask [10], [27], and the intercepts with the septa were counted.
The complexity of the lung parenchymal tissue was measured by comparing the following three parameters: the number of alveolar saccules per mm2; the relative amount of parenchymal tissue; and the inter-airspace wall distance [mean linear intercept (Lm)], which measures the distance between alveolar walls.
Airspace enlargement was quantified by measuring mean linear intercept (Lm).
To determine the differences in alveolar size, measurements of mean linear intercept (Lm) were used.
Mean linear intercept (Lm) was employed to assess for alveolar size according to Thurlbeck (1967).
