Sentence examples for linear insertion from inspiring English sources

Herpes viruses are capable of both closed circular episome formation [29] and linear insertion into host genomes, and infection of lymphocytes causes activation of RAG expression [17], [19], [31].

The linear insertion vector DNA was obtained by digesting the pInsertion-ura4-barcode library DNA with BamH I and gel purifying the 2.1 kb fragment using QIAGEN Gel Extraction kit, followed by extraction with phenol/chloroform/isoamyl alcohol (25:24:1; volume: volume: volume) and chloroform/isoamyl alcohol (24:1).

To test this assumption, fission yeast cells transformed with the linear insertion vector DNA were immediately plated on low-dose 5-FOA selective medium plates (100 mg/l of 5-FOA, the highest concentration in which the cells with a single copy of ura4+ gene can survive; unpublished observation).

For each transformation, 1 μg of purified linear barcoded insertion DNA was used to transform 50 μl of frozen KRP1 S. pombe competent cells (prepared as in [ 64]).

The 2.1 kb linear barcoded insertion vector DNA was purified from the bacterial barcode sub-libraries after digestion with BamH I enzyme and used to generate the initial transformants.

linear amplification via transposon insertion.

In the series shown in Table 2, it is clear that BVMOLepto oxidizes linear aromatic ketones with insertion of the oxygen atom to the more substituted carbon.

According to the value of polymer, we infer that selective oligomerization product and oligomer wax were obtained through cyclization mechanism, while high-molecular weight PE may be obtained through a linear chain growth alkene insertion mechanism (Cossee mechanism) [16].

While this Review was being prepared, an approach that uses linear amplification via transposon insertion (LIANTI) was reported that may prove to have less amplification bias and high (97%) genome coverage [47].

The retroviral Integrase protein catalyzes the insertion of linear viral DNA into host cell DNA.

In the opposite direction, the bacterium Escherichia coli, which contains a single circular chromosome, can be manipulated to grow with a linear chromosome by the insertion of N15 phage telomeres (Cui et al, 2007).