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IS117 was amplified from an integrated linear copy in the S. coelicolor A3(2) genomic DNA using primers IS117-KpnI and IS117-NheI (Table 1).
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This was accomplished by expressing multiple linear copies of the hinge and CH2 domains (HCH2) of human IgG1 fused to the framework region of human IgG1.
Transcription and reverse transcription are linear copying processes, with the original template removed after completion.
While extrachromosomal, frequently linear copies of the viral genome persist for extended periods of time in the nucleus of these cells, which could even allow for some viral gene expression, this does not permit the generation of viral progeny and virus spread.
The enzyme catalyzes a multi-step reaction i.e., 3'-end processing and strand transfer, to integrate a linear DNA copy (cDNA) of the retroviral genome into the host cell DNA [2], [7], [8].
Substitution of the SUS1 gene with a linear cDNA copy results in temperature sensitivity and histone H2B deubiquitination defects (Hossain et al., 2011).
Open image in new window Fig. 1 Freundlich (left) and Langmuir (right) isotherms for MB adsorption on Co3O4/SiO2 nanocomposite, The solid lines are the linear fits (copied form Ref. [57]).
Approximately 250 of these 372 CNVs are deletions for which the intensity is typically non-linear with copy number.
Successful linear enzymatic copying of one of the 81-mers was then carried out to prove that PCR amplification of the chemically modified DNA was not a rare event.
There are more than 1.8 million markers on a single 6.0 array, with half of these being non-polymorphic probes selected for their linear response to copy number and genomic position [24].
While if a plasmid is linear and single copy, ContigScape cannot distinguish it.
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