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The results revealed a good linear calibration fit in the range of 2-14 μg/ml.
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Better performance is observed for the methods that involve a nonlinear model such as γ="αtβ fitted to calibration data, instead of a single-point linear calibration commonly used.
This strategy has been produced for the pharmaceutical applications and dedicated to the preliminary evaluation of analytical methods where linear calibration model, which is very often occurred in practice, could be the most appropriate to fit experimental data.
Linear calibration curves for each compound were obtained with r2 values ranging from 0.98 to 0.99.
A linear calibration curve (Fig. 3).
The dye concentration was quantified by linear calibration with standards.
The accuracy with which the individual TTP estimates fit the log-linear calibration line can be used to estimate assay measurement error (the intra-assay variability, σ) by fitting a hierarchical mixed-effects model to multiple dilution series (allowing estimates of β0 and β1 to vary among experiments to determine whether this significantly improves the fit of the model).
The ratio [18C6 + K]+/[18C6 + Na]+ in the mass spectra of the solutions mixed from the theta-glass emitters as a function of the same ratio determined from the linear fit to the calibration curve data results in a line with a slope of 0.99 and a correlation coefficient of 0.97.
The amount of each nucleoside was calculated from the obtained area ratios and the linear fit equations of the calibration curves.
Dashed line: linear regression fit.
p < 0.001 for linear model fit.
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