Sentence examples for linear amplification procedure from inspiring English sources
500 ng total RNA was amplified using the MessageAmp modified Eberwine linear amplification procedure (Applied Biosystems).
The ds cDNA is then amplified using a linear amplification procedure.
Hybridization was performed overnight according to the Agilent, Low NA input Linear Amplification procedure at 65°C.
We developed a method to prepare and label miRNA targets using a linear amplification procedure in which an antisense-labelled target is produced.
The resulting RNA samples were amplified using a conventional in vitro transcription-mediated linear amplification procedure (MessageAmp II, Life Technologies), reciprocally labeled with AlexaFluor 546 or AlexaFluor 647 (Life Technologies) for induced allergy and control samples and hybridized to mouse whole-genome gene expression arrays (SurePrint G3Mouse GE 8x60K Kit, Agilent).
However, these methods employ either lengthy linear amplification procedures, or primer extension (4 cycles) and 15 cycles of PCR – all prior to a standard Illumina library prep entailing a further 17 18 cycles of PCR.
The established Affymetrix linear amplification procedures using the GeneChip® Expression 3'-Amplification Two-Cycle cDNA Synthesis kit yielded closely similar profiles between the five replicates of the same treatment (Pearson's correlation coefficients of the range 0.914 to 0.949), indicating a highly reproducible procedure.
Rare sequences are enriched by slower hybridization and the non-hybridized RNA is recovered and amplified by a linear RNA amplification procedure, which minimizes the biased exponential amplification of subsets of genes in a mixture.
Labelled miRNAs were prepared either by a procedure involving linear amplification of miRNAs (labelled-aRNA) or using a direct labelling strategy (labelled-cDNA) and analysed using a custom miRNA microarray platform.
The sequence specificity of UV-induced DNA damage was investigated in a specifically designed DNA plasmid using two procedures: end-labelling and linear amplification.
Following the reproducible procedures of RNA extraction and linear amplification from our previous study [ 43], we isolated total RNA from individual oocytes/embryos using TRIzol (Invitrogen, Grand Island, NY) and co-precipitated the RNA with linear acrylamide (Ambion).
