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Cells on Fn-coated PU scaffolds formed fibrillar Fn, synthesized significantly more collagen, and showed linear alignment of the secreted type I collagen when compared to cells grown on the other protein-coated scaffolds and the non-coated control.
Linear alignment of individual SWCNTs was achieved with an average diameter of ~1 nm, and a density approaching ~10 SWCNT/μm.
Briefly, Protparam [25] is a proprietary computational software available at Swiss Prot/UniProt that can be used to derive series biophysical profiles as well as determine the amino acid composition of any protein or polypeptide of interest, provided its primary structure (linear alignment of amino acids) is known.
ED-B fibronectin mRNA-positive cells seem to be concentrated in areas of fibrous stroma recruitment with a linear alignment of stromal fibro-/myofibroblasts (desmoplasia).
This example demonstrates the suitability of the linear alignment of the retention time obtained from MCC for various compounds in real samples.
We further implemented a line detection process to identify the presence of linear alignment of sequential points which could indicate periods of movement within a static cluster.
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The LC-MS runs performed on the Agilent ESI-TOF mass spectrometer were used for a comparative analysis using XCMS [ 56] to identify the most significant features after performing a non-linear alignment of the chromatograms and using a Student's t-test.
PriFi only accepts relatively short alignments and so 4,000 bp segments of the complete alignment were subjected to analysis; each segment overlapped the next by 1,000 bp and a segment including both ends of the linear alignment (to complete the mtDNA circle) was also analyzed.
For retention times of more than 25 s, the linear alignment leads to significant improvement of the correspondence of the positions of the signals in the data from both instruments.
The analyses of jacking force show that reduction in jacking force is closely related to reduction in friction coefficients, and the effect of lubrication is slightly more significant in the case of curved alignment than the case of linear alignment.
The paper also briefly discusses necessary improvements in areas of linear alignment, aspatial matching and higher-level matching.
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