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Further analysis showed that MSCs on soft substrates had weaker cell adhesion, and that the suppression of cell adhesion strength mimicked the effects of soft substrates on the lineage marker expression.
Following 72 h of culture, cells were simultaneously analyzed for lineage marker expression and CFSE fluorescence.
So to better examine cell fate, shifts in MSC lineage marker expression were monitored over time.
The colonies also expressed the bile duct lineage marker KRT19 and KRT7 (Figure 4 A and B).
Id1 expression is confined to the primitive progenitor cells (lineage marker negative, or Lin- subset) in resting BM [13].
To investigate the stomata development in AP2C3oe lines we studied the expression of stomata lineage marker proteins and gene activities.
Hence, it is unknown if other adult stem cells from skin already express lineage marker genes at a similar level.
Emr1 is the prototypical macrophage lineage marker in the mouse, although its tissue distribution appears to be different in humans [45].
In preliminary experiments, differentiating WT and PPARγ−/− cells were analyzed daily for 7 days by qRT-PCR to determine the time of maximal expression of each lineage marker.
KRT7 and KRT19, two conventional markers of cholangiocytes, were detected in the surrounding monolayer cells, whereas the hepatic lineage marker AFP was undetectable (Figure 6D and E).
At this developmental stage, hematopoietic cells (CD43+) express the progenitor marker CD34 and the erythroid lineage marker CD235a (GlycophorinA) (Fig. 2A).
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