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Duplication is characteristic of insect populations being isolated from one another while the host is not and does not imply that a duplicated lineage has to arise on the same host species nor in allopatry [ 52].
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To detect putative clinical or experimental phenotypes, assignment of individual bacterial strains to specific clades or strain lineages has to rely on phylogenetically well-defined groupings.
To get a more precise and phylogenetically meaningful background for an evolutionary interpretation, the sample of free-living bacteria as a possible source of symbiotic lineages has to be much improved.
This strongly suggests that a HAL was present in the most recent eukaryotic ancestor and the absence of HAL orthologues in some eukaryotic lineages has to be interpreted as a consequence of gene loss.
Interestingly, these lineages have, to our knowledge, not been reported in Swedish pigs before.
Under a general lineage or unified species concept, the only criterion used is that lineages have to evolve separately from other lineages to be considered species [ 7].
To initiate differentiation, stem cells have to be forced out of self-renewal, the transcriptional networks conferring stem cell identity need to be dissolved, and lineages have to be chosen.
Some lineages had to be included into imprecise groups such as H/HV/R for haplotype and haplogroup frequency comparison, although all Saudi haplotypes were completely sorted into their respective clades and sub-clades [see Additional file 1].
The longer it has been since two species diverged from a common ancestor, the more time each lineage has had to accumulate mutations.
We demonstrate that the pattern of background substitutions in the saltans/willistoni lineage has shifted to a significant degree, primarily due to changes in mutational biases.
The presence of a functional relaxin in the ruminant lineage has yet to be confirmed [ 25].
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