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RNAs from control or hypoxia-treated macrophage cell line were mixed with the spikes and hybridized to our test-array.
The two competitors, a mutant line vs the A (REL1206) line, were mixed at a 1 1000 volumetric ratio and diluted 100-fold into 10 ml of DM25.
In order to initiate the fusion process 10 cells of each distinct cell line were mixed together in a 24-well plate and left for 3 h at 37°C and the queried Rho-labelled peptides were added 30 min before the end of incubation.
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The correct treatment of the line profile, however, requires that the difference formulation in the core of the line be mixed with the standard formulation in the wings, and this may limit the advantage of the method.
One μg of RNA from each cell line was mixed with 1 μl 10 mM dNTPs, and 1 μl 250 μM random primers in a final volume of 12 μl.
The 293FT-CRP and 293FT-SAA cell lines were mixed in equivalent numbers for culture.
The A549 and RPMI8226 cell lines were mixed with wild-type DNA in serial dilutions of 50%, 25%.
The A549 and RPMI8226 cell lines were mixed with wild-type DNA in dilutions of 50%, 25 %, 12 %, 6 and 3%.
For proteomics analysis, whole cell extracts isolated separately from "light" (empty vector) and "heavy" (eIF6 over-expression) cell lines were mixed in equal amounts.
(A ) Light-labeled (K0) shFF2 and heavy (K8 -labeled shQIL1 stable cell lines were mixed at a 1:1 ratio and mitochondria subsequently isolated and lysed with 1% Digitonin.
Individual Ccnd1+/+ and Ccnd1 KI/KI lines were mixed with Matrigel and injected subcutaneously into the flanks of nude mice.
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