Three AN lines (AN1, AN2, and AN3) and the NT line were grown hydroponically under Fe-sufficient conditions.
A tobacco line genetically modified to produce two N-acyl homoserine lactones and its non-transformed parental line were grown in non-sterile soil.
Cells from the CCD1072Sk line were grown in 96-well plates with complete medium for 16 h until cellular adhesion was attained.
Forty plants of each line were grown in the experimental field of Yangzhou University (E119°25′/N32°23′), from May through October in 2016.
The Hep-2 cells (human larynx epidermoid carcinoma cell line) and Vero cells (African green monkey kidney cell line) were grown in 96-well plates (8 mm diameter; Falcon Plastics, Oxnard, CA) and incubated at 37 °C in a humidified atmosphere supplied with 5%% carbon dioxide.
Cells of the mouse C2C12 myogenic cell line were grown as described [39], [40].
Maize plants (B73 inbred line) were grown under replicated field conditions and sampled at V14-15 stage (Materials and Methods).
Four independent offspring pairs, each consisting of a GM line and its corresponding non-GM control line, were grown under different soil nutrient conditions and with and without fungicide treatment in the glasshouse.
SupT1 cells (a human T-cell line) were grown in RPMI (Gibco) supplemented with 10% FBS (Gibco), 1% Penicillin/Streptomycin (Pen/Strep, Gibco), 1 mM sodium pyruvate, 10 mM Hepes, and 2 mM L-glutamine.
A549 human lung carcinoma cell line were grown and maintained in DMEM containing 4.5 g/l glucose, supplemented with 10% Foetal Bovine Serum, penicillin (20 U/mL) and streptomycin (20 ug/mL; all Invitrogen).
HEK293 cells, a human embryonic kidney cell line, were grown in Dulbecco's Modification of Eagle's Medium (DMEM) (BioWhittaker) supplemented with 10% foetal bovine serum, 1% L-glutamine and 1% Non-Essential Amino Acid (BioWhittaker).
