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To reduce risk, the line was split into two separate phases, to be managed by Union Railways (South) and Union Railways (North).
At the fifth passage, each cell line was split into parallel flasks (three each for expression and promoter analysis, and two for Copy Number Variation/Single Nucleotide Polymorphism (CNV/SNP) analysis, and grown to approximately 70% confluence.
Subsequently, each line was split up in two treatments: coevolution and control.
Each iPS line was split from one well into the three wells, and expanded, prior to RNA isolation.
To generate the uninfected S2U line, the S2I cell line was split and treated with tetracycline (Fig. 1).
Each AD-iPS cell line was split from one well into the three wells for expansion before the RNA was isolated.
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I love that the famous Bono line is split, with Jase handing over to Matt Goss who performs swift butchery on the Bono legacy.
The Kuro line is split in two: the Kuro and the Elite Kuro.
Each transmission line is split into segments according to the disaster areas it passes by.
One hundred female Japanese quail from a commercial line were split into 20 groups of 5 individuals when 6 weeks old.
For the ATP6[1] and temperature sensitive selection, the five lines that we followed at 29°C were also followed at 22°C (Populations in each line were split into half at generation 1 and kept at either 29 or 22°C for the following generations).
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