As described in his Dream Pool Essays, Shen Kuo enjoyed the company of the "nine guests" (jiuke), a figure of speech for the Chinese zither, the older 17x17 line variant of weiqi (known today as go), Zen Buddhist meditation, ink (calligraphy and painting), tea drinking, alchemy, chanting poetry, conversation, and drinking wine.
The cell lines used in the present study include the GFP-transfected parental, poorly metastatic MDA-MB-468GFP (468GFP) cell line and a progeny MDA-MB-468GFP-LN (468LN) cell line variant that is highly tumourigenic and that also maintains a propensity to metastasize in a mouse xenograft models.
Cells from the MDA-231 breast cancer line variant, which reportedly do not express PTH1R [73], were maintained in α-MEM medium supplemented with 10% FBS and insulin (10 µg/ml) and were used as a negative control for PTH1R expression (see below).
To determine if Nab2 is required for the repression of miR-17-92 miR-17-92 miR-17-92l line variant that stably expresses an shRNA against Nab2 (shNab2 PUER) (Laslo et al, 2006).
An MDA-MB-231 breast cancer line variant, MDA-MB-231-1833, withighgh metastatic bone tropism, was treated with a novel catalytic PI3K/mTOR inhibitor, PF-04691502, at nM doses that did not impair proliferation.
We suggest that the T59K germ line variant is a low-penetrance allele with respect to tumor growth, but additional genetic variations of unknown origin may enhance the family history of cancer.
Cells with concurrent BRAF mutations and melanocortin 1 receptor germ line variants and/or a more differentiated melanocyte genotype had a preferential response.
Although no cultured mammalian cell line completely lacks topo IIα, there are cell line variants such as HL-60/MX2 cells with compromised topo IIα activity.
LOH can lead to decreased gene expression or hemi/homozygosity of germ line variants or somatic mutations affecting the remaining allele.
The set of BRCA2 samples was screened to address the possibility that germ line variants could modify BRCA2 risk.
The remaining three germ line variants of CHK2 are also detected in the normal population and are therefore putative polymorphisms.
