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NSC cultures expressed higher levels of many mRNAs previously identified as specific for human NSC, including PAX6, FOXG1B, TH, NKX2-2, CHATPH2PHDAC6DAC6, IL11RA, COL2A1 [36], [36] (including genes not found to be significantly different due to cell line to cell line variance).
The control line variance was significantly lower than the MA line variance for six fitness components (liquid growth, slug distance, total number of FB, spores per FB, spore germination, and competitive ability).
The control line variance was significantly higher than the ancestor for two fitness components (liquid growth and spore germination).
The heritability of each transcript was estimated from the pooled within line variance of the parents as in Keurentjes et al. (2007).
This is a standard approach in MA experiments, and it uses the changes in the mean MA line fitness and the among line variance to generate parameter estimates.
For seven of the eight fitness components, the prediction held, and the MA line variance was significantly greater than the ancestor.
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To test for this, we determined whether the control line variances were significantly different from ancestor and MA line variances (Table 2).
However, among-line variance may result from non-genetic factors; e.g., see figure 3 in [23].
We therefore repeated the above analyses using the raw within-line variance (VE).
Denver et al. [12] identified 686 of the 7056 genes that had accumulated significant mutational (among-line) variance (VM).
Vg and Ve were inferred from between- and within-line variance components extracted with procedure VARCOMP (SPSS Inc 2009).
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