Read more articles aByut video games on VICE here, and follow us on Twitter at @VICEGaming.
Since a subpopulation of the VE, the AVE has been shown to have heart-inducing ability, it is possible that these molecular differences might also reflect differences in the ability of these cell lines to activate and/or enhance cardiac differentiation in ES cells.
These data further support the notion that the radiosensitivity of DSB repair proficient human tumour cell lines may be partly determined by the predisposition of these cell lines to activate non-conservative DSB rejoining pathways.
These data raise the possibility that the radiosensitivity of DSB repair-proficient human tumour cell lines may be partly determined by the predisposition of these cell lines to activate non-conservative DSB rejoining pathways.
In experiments using inducible Cre lines to activate a permanent genetic label for cellular fate mapping (Kretzschmar and Watt, 2012), Cre toxicity might affect the longevity, proliferation and differentiation of cells, which in turn could influence the results.
We asked if using the split-GAL4 lines to activate or inactivate neurons would perturb general innate behaviors, such as locomotion and visual perception that might interfere with our assays of memory, locomotion and sleep.
DOI: http://dx.doi.org/10.7554/eLife.04402.003 We first used these Gal4 lines to activate specific enteric neurons by expressing the temperature-sensitive ion channel, TRPA1, and measured the effects on hemolymph glucose levels (UAS-TRPA1 [ Hamada et al., 2008]).
The R26B05 line used to activate the lineage 11 cells occasionally showed expression in a bundle of descending interneurons and we were concerned that their severed axons might be responsible for driving the takeoff behavior in the decapitated flies.
As expected, the antagonistic activity of LBD antibody WC613 was strongly dependent on the particular ligand-expressing cell line used to activate Notch1 (Figure 3A).
Ethograms of the scored behaviors are displayed as described in Figure 2. DOI: http://dx.doi.org/10.7554/eLife.02951.012 10.7554/eLiFigure51.013 Figure 2 figure supplement 2. Anatomy of GAL4 lines used to activate distinct cleaning movements.
