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Here we report the generation of a mouse line that lacks CgB (CgB-ko).
This allowed using anti-IgG antibodies as an alternative trigger for BCR activation in a cell line that lacks IgM but exposes IgG, as is the case with SUDHL4.
A. Egfr f24 prefollicle cell clone (indicated by dashed yellow line) that lacks pErk signal (red).
These molecules were expressed in the A20IIA1.6 (IgG2aκ) mouse B cell line that lacks endogenous FcγRIIb expression.
For this purpose, we studied wild-type CHO-K1 cells and 10 3 cells, a mutant CHO-K1 cell line that lacks NPC1.
To facilitate this reconstitution, we looked for a cancer cell line that lacks cavin-3, but expresses normal levels of all other linkage components.
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SHIV-1157ipEL did not replicate in any cell line that lacked CCR5 (Fig. 2B; Methods).
Interestingly, the Ln229 cell line that lack measurable levels of the HGF/c-MET complex in the FFPE assay (Fig. 7B) exhibited elevated levels of c-MET (pY1003) phosphoryaltion in the lysate immunoprecipitation assay (Fig. 7C).
MiaPaCa-2/CD70 cells were effectively killed by SGN-75 in vitro with an IC50=29 ng ml−1, whereas the parental cell line that lacked CD70 was unaffected.
Aversive memories were formed when activating TH GAL4 neurons but not with a GAL4 line that lacked expression in a cluster of dopaminergic neurons called Protocerebral Posterior Lateral 1 (PPL1).
In the parental 231 line that lacked high cytoplasmic p27 (Fig. 3b), p27 knockdown did not significantly affect motility and invasion.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com