Our cell line, termed SS-iASC, has been characterized by karyotyping and cell line identification, and stable expression of SS18-SSX1 has been verified using real-time PCR (RT-PCR), nested PCR, immunofluorescence, and immunoblotting.
This transgenic line, termed Pdx1PBHnf6, was maintained on an inbred hybrid (B6D2) background.
Recently, we established a novel type of human Treg cell line, termed HOZOT, multifunctional cells exhibiting a CD4+CD8+ phenotype.
Next, we tested female fertility and egg permeability employing an independent RNAi line, termed M6-RNAiVDRC, which was also directed to the FE.
This resulted in a transgenic line termed 3D7attB::PfSCD-GFP, in which the Pfscd-gfp sequence was integrated into the chromosomal attB site (Fig. 3A).
The majority of experiments were conducted with a line termed in[act:FoxO-GFP]1, in which a subset of cells of the interstitial lineage expressed the fusion protein.
Similar to our work, Duffy et al. [11] have generated var2csa-disruption mutants in a parasite line, termed CS2, which is isogenic to the FCR3 parasite used here.
A multifunctional Treg cell line, termed HOZOT, was generated by co-culturing human umbilical cord blood cells with mouse stromal cell lines in the absence of exogenous IL-2 or other cytokines [16].
Additionally, we established a drug resistant 762TuD cancer cell line (termed res. cell line).
A line termed P. inflata-1 was derived from seed originally received from Ken Sink (Michigan State).
