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The rationale was to create a cell line tagged with at least two docking sites.
With lines obtained from CSHL, we observed reporter gene expression from lines tagging 2 of 3 genes.
All CPTI lines tagging these genes are later enriched in the CNS in late embryogenesis (supplementary material Table S1).
The newly developed protocol was then used to sort stable populations of both cell lines tagged with a DsRed-expressing cassette.
Other lines tag two kinases CaMKII (CPTI-000944) and Drk (CPTI-002249, note that the main localisation is in nuclei).
Two algorithms will be proposed, including Trail-based Algorithm with virtual Guide line (TAG) and enhanced Trail-based Algorithm with virtual Guide line (eTAG).
In brief, suppressor line TAG-11 (var2-5 svr3-1) was crossed with Landsberg erecta to generate an F2 mapping population.
Both post and comment in-line tagging are available on the mobile site and Android.
Snags the line drive, tags the runner and makes the throw to the plate.
The second advert features the tag line "take, tag, sort and share.
This makes Wilson an essential handcuff for Bradshaw owners, much in the way Brandon Jacobs was last season (without the goal-line tag).
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