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Due to segregation in the heterozygous SBE RNAi4.9 line starch from only transformed grains was purified and studied separately.
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In the super-transformed lines, starch content and tuber yield were about the same as in the control.
In contrast, starch granules from line 1047 17 exhibited a huge amount of gelatinisation, producing a large and diffuse pellet with a swollen volume 3-fold greater than that of wild-type.
By contrast, starch content in line K decreased to 11.8 % after 36 weeks of storage, compared to a greater, almost unchanged mean value of ~14 % in the OCI-expressing tubers (P = 0.002, F = 19.40).
In the durum wheat lines, total starch was 61.8% in the parent, 62.3% in wild-type sibling lines and 55.2% in mutant siblings on a dry weight basis (Table 5).
The company's product line includes starches and sweeteners, animal feed products and edible corn oil.
In these null SSIIa wheat lines, resistant starch was also reported to increase from wild-type levels of 0.02% up to 3.6% [ 22].
We found that the high amylose SBEIIa TILLING mutant lines formed starch granules with reduced birefringence and had a high proportion of crescent shaped granules similar to those reported for high amylose hp-SBEIIa RNAi lines in wheat [ 28].
Although we infiltrated in a restricted region of the leaf (outlined in Figure 5C and 5D with dotted lines) dark starch staining was not limited to the infiltrated region, but spread to adjacent tissue, implying the spread of the hormone.
Homozygous mutant lines stained for starch using Lugol solution showed an increase in starch accumulation (data not shown).
In most of the transgenic lines inhibition of starch synthesis was accompanied by increased hexose-6-phosphates (Table 2).
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