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Database searches revealed the homology in LINE sequences in closely related genera Arabidopsis indicating their origin from common ancestors predating their separation.
We used RepeatMasker to search for LINE sequences in the contiguous segments.
Despite the comparable levels of genome occupied by LINE sequences in human and mouse [ 12, 13], there are more than 15 times as many full-length L1 elements with intact open reading frames in the mouse genome [ 20].
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The insertion site of Bov-A2, though different in Antilopinae and Tragelaphini, was within a LINE sequence in the TP53 promoterter, approximately 500 bp and 460 bp upstream to the TSS, respectively.
However, we caution against any functional inference, since these enrichment patterns could simply reflect the presence of CENP-A over a region of the CarSat1 array that happens to contain amplified LINE sequence in the particular canine genome tested here.
Expression of mouse Foxg1 was also restricted to brain tissue and brain related cells, but surprisingly the two novel TSSs of mouse Foxg1 were also found highly expressed in the single mouse cell line sequenced in the FANTOM5 project (fibroblast cell line) suggesting that other than the brain, fibroblast cell lines may be useful for in vitro analysis of Foxg1 in mouse.
Several studies have subsequently examined Chk2 germ-line sequence in families with hereditary breast cancer.
The lines sequenced in this study were chosen to represent the diversity within the more comprehensive validation panel.
CNVs were detected in three of four cell lines sequenced in our sample cohort and confirmed by the MIP array-based CNV detection assay or FISH analysis (Supplementary Table 8).
In contrast, the average proportion of LINE sequences was significantly lower in CNV-type core elements than in the constant type.
In addition, a 1.2 kb fragment of SsaRT.3, a non-LTR long interspersed nuclear element (LINE) sequence identified in AS (unpublished data), is inserted approximately 1420 bp 5' of the transcription start site in CS gh1.
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