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That awkwardly straddled line of wild and not-wild will be felt here on the Wilson place.
One of the most popular quotes is the last line of Wild, "How wild it was to let it be".
For all other experiments, a cloned line of wild type P. chabaudi AS strain was used in this study.
WSL-R line 379, a macrophage cell line of wild swine origin that was kindly provided by Günther Kiel (Friedrich-Loeffler Institut, Greifswald, Germany), was grown in Iscove's medium (Gibco-BRL, Grand Island, NY, USA) plus F-12 nutrient mixture (Gibco) plus 10% FBS.
Remember, even docile house pets actually come from a long line of wild cats, and it's in their DNA to want to hunt, chase and pounce.
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To validate these findings in an independent system, we introduced DOX-inducible KLF2 and NANOG transgenes in a line of wild-type WIBR3 human ESCs.
The ILs were developed by introducing single chromosome (chr) segments from SS18-2, a line of wild-like weedy rice (O. sativa), into the recipient genetic background.
The results of this transgenic mouse study were obtained through analysis of a single line of wild-type WNK4 transgenic mice, and whether WNK4 protein abundance was indeed increased in the kidney was not shown.
Remarkably, in one line of wild-type VAPB overexpressing mice we observed a variant of stacked ER where the cytosolic space linking the cisterns was considerably more electron dense.
In Drosophila loss-of-function mutations in the LKB1 gene caused defects in polarity of the oocyte, and this was rescued by low level expression in the germ line of wild-type LKB1, but not by mutation of the residue homologous with Ser (Ser) [ 29].
Drosophila loss-of-function mutations in the Lkb1 gene caused defects in the polarity of the oocyte, and this was rescued by low-level expression in the germ line of wild-type LKB1, but not by mutation of the residue homologous with Ser (Ser) [ 29].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com