This cell line, irrespective of passage level, is 100% GFAP positive.
The second group was explicitly prompted to read each line of the passage and then explain to themselves the meaning of the text before proceeding to the next line, and to repeat this for each line of text.
Primary cell lines of low passage number (2 3 passages) were infected with the VSV-G pseudotyped retrovirus particles in the presence of 1 µg/ml polybrene (Sigma).
In contrast to cell lines of high passage [ 31, 32], low-passage cancer cell lines well reflect the biology of the original tumor, such as growth behavior, morphology, and mutational profile and are, therefore, in our experience, a versatile tool to evaluate drug efficiencies in a preclinical context.
But with a simple analysis, we find that this process is in line with rite of passage.
Both theoretical analysis and case study have shown how difficult it is to draw a line between rite of passage, ritual, and everyday behavior.
Additional transverse marks, at 3 cm and 5 cm proximally and another mark at 3 cm distally to the tenotomy line indicated the transverse and oblique lines of suture passage (Fig. 1c).
Cell lines of higher passage numbers are thus likely to contain less mutant mtDNA (van de Corput et al., 1997).
Sailing over major latitudinal lines is a right-of-passage that involves a secret initiation ceremony, most especially for equator crossings.
Some in the tech industry were quick to line up in favor of passage.
Chromosomal analysis of the most stable line at passage 41 was added at 60 passage.
