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To complete the product line, Null has offered this THUMP exclusive mix.
Unfortunately, the mixed lines are not suitable for behavioral tests, as only a few 129sv/C57BL6J back line null mutant mice for Scrapper survived until adulthood [1], [30].
We utilized a previously described Saos-2 cell line (null for endogenous p53), which contains a tetracycline-responsive p53 transgene.
There was no difference in Na+ or K+ concentration for any treatment between aneuploid line null for the TaHKT2 1 7DL-1 gene and Chinese Spring.
The dotted horizontal red line ("Null of zero effect") is the distribution expected if there is no effect in the data.
PBC-value > 0.05 indicates that nodes with high betweenness-centrality in the disease/process network do not significantly correlate with the genes mutated in the cell line –null hypothesis.
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Aneuploid lines null for TaHKT2 1 7AL-1, TaHKT2 1 7BL-1 and TaHKT2 1 7BL-2 showed no difference in Na+ concentration between Chinese Spring except for higher Na+ in sheaths.
Aneuploid lines null for each functional gene were grown in high NaCl nutrient solution culture to identify potential role of each TaHKT2 1 member.
The use of wheat aneuploid lines null for specific members of the TaHKT2 1 family provided a system to analyse phenotypic effects of the genes on tissue ion concentrations.
An investigation of possible phenotypic effects of TaHKT2 1 genes was conducted by evaluating tissue Na+ and K+ concentrations in wheat aneuploid lines null for each member of the TaHKT2 1 gene family, when exposed to NaCl and different K+ supply in the external solution.
We used four wheat lines carrying the transgene Pm3b in different position on the genome and their respective non-transgenic control lines (null-segregants), each derived from different transformation events [18], [19].
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Justyna Jupowicz-Kozak
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