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When M-SAA was expressed from the psbA promoter in a non-photosynthetic strain (that is a line lacking the psbA gene) the protein accumulated to 3% TSP.
The immune response was demonstrated to be Her2/neu-specific since Her2MPyVLP immunization did not induce rejection of the parental D2F2 cell line (lacking the transfected Her2/neu gene) [8].
The basic design to study the substrates of transporters is to determine the uptake of a selected test substance into a cell line that expresses the transporter of interest in comparison to the same cell line lacking the transporter.
The establishment of a human erythroid cell line lacking the genes to produce the A, B, and RhD antigens would be a universal resource for clinical application, since the cell line produces O/RhD RBCs which are transfusable into all individuals in theory.
Moreover, the fragment from TtPMM-B2 was absent in the LDN substitution line lacking the 4B chromosome (Additional file 7).
Among these, five kinases show an elevated protein level after geldanamycin treatment compared to a cell line lacking the mutation.
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Lincoln's product line lacks the risky styling and performance-driving orientation of its crosstown rival Cadillac, but Lincoln recently has experienced successes on some fronts.
Even the straightforward parodies of classic Hindi musical numbers, in which love usually dances with the chaperonage of a chorus line, lack the comic spark to send them soaring.
The Vero E6 cell line lacks the ability to transcribe type I IFNs as they possess a genetic deletion that ablates the entire type I IFN gene complex on both chromosomes [9].
For example, CHO-cell-derived epoetins display low levels of N-glycoylneuraminic acid which is not present on sugars attached to Epoetin delta because the human cell line lacks the necessary enzyme and monosaccharide to carry out this step.
It needs to be mentioned that this transgenic line lacks the presence of trichomes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com