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Wnt proteins are also thought to play a role in the development of the lateral line in zebrafish (reviewed in [53]) and chicken inner ears [54], [55], [56].
During development of the lateral line in zebrafish, cells migrate collectively along the body of the embryo and deposit cell clusters called rosettes at discrete locations; these are the progenitors of mechanosensory organs [ 50].
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Here we describe a system for chemical-inducible gene expression and demonstrate its feasibility for generating transgenic driver lines in zebrafish.
Moreover, the available number of Cre-lox lines in zebrafish is currently limited and restricts the application of these systems.
However, it is important to consider that the existing tetanus toxin transgenic lines in zebrafish are not expressed in a sufficiently strong way to address it.
Recent efforts to generate tardbp knockout lines in zebrafish have highlighted an unexpected subtlety associated with the use of this model organism.
A recent systematic analysis reveals poor phenotypic correlation between published Morpholino-induced morphants and mutant lines in zebrafish [ 4], further highlighting the off-target effects of Morpholino oligomers.
This was done in an effort to circumvent variegated transgene expression resultant to methylation-induced silencing of UAS-based transgenic lines in zebrafish, specifically demonstrated to effect expression of the Tg(14xUAS:nfsb-mCherry c264 nfsb-mCherry c264
Apart from in vitro studies, miRNA mutant and transgenic lines should be explored as these lines in zebrafish have widened our understanding of the molecular processes that govern the phenotypic outputs of several transcriptional factors, signaling molecules, and genes.
On the basis of these literatures and our current results, we propose a model of Mil mechanism underlining the development and survival of hair cells and neuromast of the lateral lines in zebrafish.
For example, during both Primordial Germ cells (PGC) migration and lateral line formation in zebrafish, Cxcr7 has been proposed to act as a decoy receptor that sequesters sdf1 ligand making it unavailable for Cxcr4 [30], [30].
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