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Once gene lists were identified for the toxicant-specific responses of each cell line, hierarchical clustering analysis was conducted using the program Cluster (version 2.0; http://rana.lbl.gov/EisenSoftware.htm) to perform uncentered, average-linkage clustering; the data were visualized using Treeview (http://rana.lbl.gov/EisenSoftware.htm; Eisen and Brown 1999; Eisen et al. 1998).
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By consistency analysis within each line using hierarchical clustering of the total expressed genes, heterogeneity among individuals in each line was demonstrated.
In order to make cell lines for hierarchical screening, we have raised immortal cell lines from the ureteric buds of embryonic kidneys of 'Immortomouse' embryos.
Array-based methylation analysis was performed for EDK, iPDK and their parental hES and iPS cell lines, and hierarchical clustering revealed that EDK and iPDK had closely-related methylation profiles.
Phylogenetic trees are constructed from nested groups of taxa, and from a cognitive perspective, synapomorphies help identify points along continuous lines where hierarchical levels begin.
Among the tamoxifen-resistant cell lines, unsupervised hierarchical clustering revealed very similar expression profiles (Supplementary Figure S1, 15 376 genes) and did not group the bulk of cell lines belonging to a particular cVIS (Van Agthoven et al, 2009b).
Thirty-four transcripts (34/290, 11.72%) were significantly altered in melanoma cells when compared to normal cells (Table 2) and could clearly distinguish normal cells from the melanoma cell lines in hierarchical cluster analysis.
James Graham, the social media director for the campaign website, has described the campaign as a living nightmare, saying it was run on "rigidly hierarchical lines and secretive lines".
Thus, different segments of a line depict different hierarchical levels of relationship rather than the entire line depicting a single hierarchical level.
These results are more in line with the hierarchical view.
The resulting problem is referred to as assembly line balancing with hierarchical worker assignment (ALBHW).
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