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RESULTS: DU 145 cell line exhibited a missense mutation in codon 84 (GAC to TAC).
Especially, after optimization via AGRO100 and HA2, GS-PEG/HA2-Apt in each cell line exhibited a synergistic effect on transfection efficiency.
The empty vector-transduced 3T3 line exhibited a consistent doubling rate of approximately 24 hours until reaching confluency between 72 and 96 hours (Figure 2 C, gray diamonds).
Neither add-back cell line exhibited a doubling time in in vitro culture that was significantly different to either the knockout or parental cells.
Gynoecia from this line exhibited a split-style phenotype similar to the spt-12 mutant.
Likewise, the napin- IMT line exhibited a similar manner of protein expression at 25 DAP.
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The mice of this line exhibit a long asymptomatic phase, reminiscent of the disease course in ALS patients.
Nine plants of each line exhibiting a segregation ratio consistent with an insertion at a single locus were transferred to soil and self-propagated.
This cell line exhibits a CD44+/CD24- profile and strong expression of SNAI2 and TWIST1.
Despite originating from the same parental cells each line exhibits a distinct gene signature (Additional file 4: Figure S2).
The M-1 line exhibits a stronger eye degeneration phenotype than the M-101 line, and it expresses ∼2.5 times more transcript in late third instar imaginal discs.
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