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Particularly, an immediate and a late-phase activation of ERK were observed during monocytic cell line differentiation.
However, a trans-germ line differentiation via ectodermal or endodermal lineages including maturation into hepatocytes, cardiomyocyte or neuronal phenotypes remains controversial [ 31].
Although immunohistochemical analysis showed a significant correlation between FAK overexpression and poor tumour differentiation, there was no relationship between FAK expression and cell line differentiation.
In Caenorhabditis elegans, PHB proteins were showed to be essential during embryonic development and are required for somatic and germ line differentiation in the larval gonad [ 51].
However, it is also possible that RNAi depletion early in germ line differentiation caused another defect that is responsible for this later phenotype.
Metastable epialleles show variable expression among individuals with epigenetic profiles that are consistent across tissues and are thus likely to have been set prior to germ line differentiation [ 21- 23].
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From each time point and each cell line 3 technical replicates were measured (combination of three cell line differentiations GSE2972, GSE3749, GSE3231).
As a large number of research groups have begun to compare the differentiated progeny of multiple iPSC lines, differentiation variability has become an issue of paramount importance.
Guidelines are presented for the observer gain design when it is used for on-line differentiation.
Furthermore, compound 1d could induce NB4 cell lines differentiation efficiently.
Possible benefits include: improved localisation of parenchymal consolidations (anterior versus posterior), detection of pneumothorax or pneumomediastinum patients in the supine position, verification of the correct position of drains and lines, differentiation between pleural effusions and consolidations, and other applications.
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