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Xia et al used ChIP-chip and RNA expression profiling of HepG2 (hepatocellular cancer cell line) and U87 (glioma cell line) cells grown in normoxic (ambient) and hypoxic (0.5% O2 for 4 hours) conditions to define HIF-1 chromatin binding targets.
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Shocked cells were recovered by adding 1 ml of SOC medium and by incubating at 37°C for 90 minutes in a lab line "Cell-Grow" tissue culture rotator.
In the HER2+ luminal cell line BT474, cells grow in tightly packed epithelial colonies.
Clonal cell lines were derived from cells grown in absence of Zeocin by limiting dilution.
For each cell line, asynchronously growing cells, or cells treated with nocodazole to enrich for the G2 M phase population, were analysed by western blotting.
For differentiation of mouse embryonic stem cell line OG2 cells were grown without LIF.
Each lentivirus was transduced in two independent fibroblast cell lines and each cell line was grown in duplicate.
A549, MCF-7 and Saos-2 cell lines were grown in DMEM, the SKBr3 cell line was grown in McCoy's and the HL-60 and Jurkat cell lines were grown in RPMI.
Cells of the mouse C2C12 myogenic cell line were grown as described [39], [40].
All three cell lines were grown in McCoy's 5A media and the HCT-116+ch3 HCT-116+ch3as grown under selecelln with 0.4 mg mlinegeneticin.
They will, however, be allowed to do research on new stem cell lines grown in a privately financed lab.
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