Three-dimensional stacks of 2048 x 2048-pixel images were acquired using a 0.16-µm step after 4x frame average and 2x line accumulation with a 2.4x or 4.74x zoom in order to get the best resolution and the best signal-to-noise ratio.
Based on mutation rate data from D. melanogaster, scaled to the slightly larger D. serrata genome size [0.215 pg (Gregory and Johnston 2008)], we predicted an average per MA line accumulation of 34 mutations, composed of 19.6 [confidence interval: 16.8; 22.8] single-nucleotide mutations (Keightley et al. 2009) and 14.8 [C.I.: 3.4; 52.2] indels and complex mutations (Haag-Liautard et al. 2007).
In glioblastoma cell lines, accumulation of PHD3 protected tumour cells against hypoxia-induced cell death via control of HIF [ 77].
Loss of viability following TS inhibition occurs as a consequence of depleted dTTP pools and at least in some cell lines, accumulation of dUTP and subsequent misincorporation of uracil into DNA.
At baseline, CAP1/ Prss8 deficient mice had no sign of alveolar epithelial fluid lining accumulation, consistent with previous observation that moderately-decreased ENaC function in AEC is sufficient to maintain normal lung fluid balance under basal condition (Egli et al, 2004; Randrianarison et al, 2008).
The timing of starch synthesis was not affected in the Atss2, Atss3 line, as accumulation was noted at the end of the light phase.
Compared to the parent line, platinum accumulation following exposure to equimolar concentrations of cisplatin was on average (across the entire concentration range) 2.9, 3.6 and 4.8-fold lower in the 41McisR2, 41McisR4 and 41McisR6 cell lines, respectively.
In the mutant line, the accumulation of the corresponding intact mRNA decreased strongly 94-999% decrease, see Additional file 2), suggesting that the T-DNA insertion altered dramatically transcription and/or mRNA stability.
This LINE-1 centromeric enrichment is uncommon among mammals, for which there is a preferential LINE-1 accumulation on positive G-band regions throughout autosomal length, and especially in sex chromosomes.
In these cell lines, the accumulation of p16INK4A was noted as another important mechanism that contributes to replicative senescence in these cell lines [18].
Glaciers in intermediate climates have intermediate equilibrium-line altitudes, accumulation or ablation totals, and activity indices.
