In fact, the limitations of this method coming from time consuming procedures, limited throughput and the complex physical phenomena of unknown cell properties (varying DEP forces as a cell moves make it difficult to quantify).
"Sprint reserves the right to limit throughput speeds or amount of data transferred and to deny, terminate, modify, or suspend service if usage exceeds 5GB per month in total or 300MB/month while off-network roaming.
The highly active, functionally diverse gene libraries generated by molecular breeding have extended directed evolution to a plethora of proteins for which only limited throughput screens are feasible.
More importantly, since numerous users are in the coverage area of each macrocell, any single user equipment (UE) gets only a small share of network resources, limiting throughput.
"Effective in 30 days, Sprint reserves the right to limit throughput speeds or amount of data transferred and to deny, terminate, modify, or suspend service if usage exceeds 5 GB/month in total or 300 MB/month while roaming off network".
In the limit as the signal-to-noise ratio approaches infinity (in which each decoding attempt requires only one iteration), however, the throughput for both the standard schedule and the IPC with confirmation is approximately 140 Mbps, whereas the limiting throughput for both the naive IPC and the IPC with stability check is nearly 180 Mbps.
In contrast, most non-nuclear two-hybrid assays and PCAs rely on a single readout, thereby limiting throughput and increasing the incidence of false positives.
These factors limit throughput at preparative scale.
Nevertheless, the approach requires complex fabrication protocols that limit throughput and multiplexing capabilities.
