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One possible factor that limits sensitivity to FMDV is the lack of expression of surface integrins, the primary class of cell receptor used by FMDV to initiate infection.
Collision induced dissociation (CID) often yields extensive compound fragmentation with structurally unique information that aids identification but limits sensitivity.
In addition, researchers should consider transcript abundances when possible while designing code sets, since the total number of counts limits sensitivity.
In agreement with mRNA levels, TGFβ1 concentration was below the detection limits (sensitivity 4.61 pg/ml) in Act A-LC supernatants, whereas Activin A was induced at 1.05 ng/106 cells (n = 5, n = 6) in TGFβ1-LC.
Optimizing specificity at 90% sets the cut point at 2.2 but limits sensitivity to ∼40%.
Although electron microscopy is highly specific for microsporidiosis, the number of organisms within a biopsy specimen limits sensitivity.
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Confirmatory diagnosis with microscopy has limited sensitivity and molecular techniques such as PCR/qPCR are available only in limited centers.
However, although NMR can yield valuable information on molecular structure, it has limited sensitivity.
However, this two-step approach has limited sensitivity and specificity.
However, these tests have limited sensitivity and specificity for the detection of renal dysfunction [5].
Then, the linearity, detection limit, sensitivity, reproducibility and stability of the sensor were also investigated.
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