The rainy day before, at the new Frank Gehry-designed Art Gallery of Ontario, for instance, I had spotted, above a large and accommodating stand with many slots for many umbrellas, a printed sign reading "Unsecure Umbrella Station!" A useful convenience with a warning about the limits of useful conveniences: your umbrella could pass in a minute, with only yourself to blame.
The plunge-frozen cells were, however, at the limit of useful thickness for ECT (500 800 nm), so data could not be collected at tilt angles greater than ∼55 60° and the resolution of the reconstructions was correspondingly anisotropic.
The recent excellent essay by Robic (2010) awakened an old concern of mine regarding whether "sequences," be they protein or nucleic acid, have escaped the limits of their useful abstraction and acquired the status of a nonexistent physical reality.
These methods look at defined chromosomal regions, demonstrating why using only karyotyping data limits the amount of useful information to be gained.
Typical printers can only print with one material at a time, which severely limits the variety of useful items that can be produced.
This finding significantly limits the range of useful Xaa, Zaa and Baa substitutions, thus facilitating the search for optimal sequences for protein engineering applications [E. Kopera, A. Belczyk-Ciesielska, W. Bal, PLoS One 7 (2012) e36350].
This bombardment prompted retaliation by Argentine fighter-bombers flying from the mainland 400 miles away, near the limit of their useful combat range.
For the Pt/SiO2 catalyst EUROPT-1, the lower limit of the useful wavelength range in the DRIFTS spectra is 1400 cm−1, compared with 1300 cm−1 in transmission.
The far western analysis of 2DE separated 4E-BP1 phospho-forms presented in Figure 1C is apparently at the limit of its useful range of detection.
But during the students' flight an electrical connection to the speaker failed, limiting the amount of useful data they could collect.
These problems increase the amount of missing data in the final aligned matrix, ultimately limiting the amount of useful transcriptomic data for phylogenomics.
